Cell Culture Medium Raw Materials

Relying on amino acid technology, cell culture medium raw materials are mainly divided into two categories: cell culture media for biopharmaceuticals and stem cell/pluripotent stem cell culture media. Focusing on animal-free, chemically defined, high-density culture and high expression, they are the mainstream raw material system in the fields of biopharmaceuticals and cell therapy.

I. Core Product Lines and Positioning

1. CELLiST Series (Specialized for Biopharmaceuticals/CHO Cells)

Targeting cell lines such as CHO-K1/CHO-S/CHO-DG44/CHO-GS, it is used for the production of antibodies, recombinant proteins and other biopharmaceuticals, meeting the standards of cGMP grade, animal-free and chemically defined.

*Basal Medium (Basal)

BASAL 3: A universal CHO basal medium, suitable for most CHO cells and supporting high-density growth.

BASAL 10: A high-nutrient density formula, suitable for cell lines with high expression/high metabolism requirements.

BASAL CHO MX: Thymidine/hypoxanthine-free, suitable for GS selection system; does not contain L-glutamine and sodium bicarbonate, which need to be added separately; contains 6.2 g/L glucose and poloxamer, suitable for suspension culture.

*Feed Medium (Feed)

FEED 2: A universal feed, which is matched with BASAL 3 to improve viable cell density and protein yield.

F7: A highly concentrated feed, suitable for long-term culture and high-expression processes.

*Special Supplements

Cys1/Cys2: Cysteine supplements, solving the problem of cysteine oxidation/insufficiency in suspension culture.

Gly-Tyr: Glycyl-L-tyrosine dipeptide, improving the stability and utilization rate of tyrosine.

2. StemFi  Series (Stem Cells/Regenerative Medicine)

Targeting iPS/ES/MSC, it is clinical grade (PMDA certified), animal-free and chemically defined, supporting single-cell passage and karyotype stability.

StemFit Basic03/04: iPS/ES basal medium, optimized formula by Shinya Yamanaka's team, suitable for the maintenance and expansion of pluripotent stem cells.

StemFit MSC: Specialized for mesenchymal stem cells, supporting clinical-grade MSC expansion.

Supporting Matrix: Laminin-511, an animal-free adherent matrix, suitable for serum-free adherent culture of stem cells.

II. Core Raw Materials and Technical Advantages

1. Composition of Core Raw Materials (Taking CELLiST as an Example)

Amino Acids: High-purity L-amino acids (including essential and non-essential), animal-free, optimized ratio, supporting efficient protein synthesis.

Carbohydrates: Glucose (4.5–6.2 g/L) as the main energy source; some contain galactose/fructose, suitable for metabolic regulation.

Vitamins: B vitamins and fat-soluble vitamins, chemically defined, without serum batch differences.

Inorganic Salts: NaCl, KCl, CaCl₂, MgSO₄, etc., maintaining osmotic pressure and ion balance.

Buffering System: HEPES/sodium bicarbonate, stable pH (7.2–7.4); some do not contain sodium bicarbonate, suitable for different culture environments.

Protective Agent: Poloxamer (Pluronic F-68), reducing shear force damage and suitable for suspension culture.

No Additives: No animal-derived components, no hydrolysates, no unknown components, complying with pharmacopoeia and application requirements.

2. Technical Barriers

Precise Amino Acid Ratio: Based on AminoScience, optimizing the ratio of amino acids to improve cell viability and expression level.

Chemically Defined (CD): All components are traceable, reducing batch differences and facilitating process verification and application.

Animal-Free (AOF): Avoiding the risk of viruses/mycoplasma, suitable for clinical applications of biopharmaceuticals and cell therapy.

High Density/High Expression: CELLiST can support CHO cell density > 10⁷ cells/mL and antibody expression > 5 g/L.

III. Application Scenarios and Selection Suggestions

1. Biopharmaceuticals (CHO Cells)

Conventional Process: BASAL 3 + FEED 2, cost-effective, suitable for most CHO cells and antibody projects.

GS Selection/High Expression: BASAL CHO MX + F7, suitable for GS system and supporting high-expression processes.

Suspension/Serum-Free: The entire series is suitable, no additional serum/adherent factors are needed.

2. Stem Cells/Regenerative Medicine

iPS/ES Maintenance: StemFit Basic04 + Laminin-511, clinical grade, supporting single-cell passage and karyotype stability.

MSC Expansion: StemFit MSC, animal-free, suitable for clinical-grade MSC production.

Ⅳ. Product Features

Core Technology	Precise Amino Acid Ratio (AminoScience)
Composition	Animal-Free, CD, No Hydrolysates
CHO Compatibility	Suitable for All CHO Series and GS System
Clinical Compatibility	Supporting IND/BLA, Small Batch Differences
Customization	More than 40 Custom Pipelines, CMO Services

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